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Description
At pH 9, CVA21 was eluted at 300 mM NaCl, while at pH 7, CVA21 was partially eluted at 1,000 mM NaCl and in the subsequent 1 mM GSH elution buffer
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Cell transfection The endotoxin-free plasmids were prepared with plasmid miniprep plus purification kit (DP01-Plus-300, GeneMark), purified by ethanol precipitation, and subjected to cell transfection
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For instance, high doses of vitamin C may increase the risk of kidney stones, especially for those with pre-existing kidney conditions
