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MRNA and miRNA expression patterns associated to pathways linked to metal mixture health effects

After incubating for 24 h at 37 C, the cells were washed with PBS and stained with Hoechst 33342 (Thermo Scientific), following the standard staining protocol provided by BioLegend

Using comparative proteomic analyses, measuring oxygen consumption, and enzymatic determination of NADH dehydrogenase, we showed that both amoebas respond to copper deprivation by upregulating the components of the branched electron transport chain: the alternative oxidase and alternative NADH dehydrogenase

It is worth noting that several studies have shown that other substance use disorders also exist with impaired brain glucose metabolism

(PubMed) Stagnaro-Green A, Abalovich M, Alexander E, et al