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To quantitate the mRNA expression levels of genes encoding transporters, heavy metal chelators, and antioxidative enzymes, the quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted using a Rotor-Gene Q PCR cycler (Qiagen) and QuantiTect SYBR Green RT-PCR kit (Qiagen) as described previously (Kim and Kang, 2018)

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Oral forms may work well for gut-related issues since the peptide contacts digestive tissues directly

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A diluted solution might not have the intended effect, leading to inaccurate data and potentially inconclusive results