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Moreover, the oral administration of carvacrol EO for two weeks has been shown to enhance the expression of key tight junction proteins such as Occludin , Claudin-1 , Claudin-5 , ZO-1 , and ZO-2 in the intestinal mucosa [186]

Proximity ligation assay Protein interactions in astrocytes were detected using the Duolink PLA assay kit (Sigma-Aldrich, DUO92101) following the manufacturers protocol

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