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We repeated this manipulation every day or every other day, until the end of the animals life

The settings of the Aixplorer scanner included a shear modulus mapping frequency of 12 Hz, the musculoskeletal mode for shear wave elastography, the penetration mode for the USWE option, an opacity level of 85%, a gain of 70%, a smoothing level of 5, persistence off, and a shear modulus of 0300 kPa