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As additional controls for filtering the selectivity of the BioID1 proximity proteomes we generated HeLa lines expressing a soluble version of cytosolic BioID1 and a version of BioID1 directed to the cytoplasmic facing mitochondrial external membrane using the TOM70 signal sequence (TOM70-RFP-BioID1) (Fig

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Keep stored in an airtight jar in the refrigerator

Quality of the peptide matters more than quantity