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For parameter estimation, the maximum-likelihood approach is used

Know what you are dealing with before choosing a treatment approach

The solvent was dried by heating, and the formed lipid film was hydrated with 0.5 mL of PBS solutions of Mb at 40 C for 30 min and then sonicated in a water bath with 100% output power for 30 min, followed by filtration through a 0.22 m syringe filter (Millipore, USA) to remove the large MLVs

doi: 10.1016/j.ymthe.2019.02.001