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For example, the coding region for mScarlet 41 was obtained as a synthetic gene whereas the one for Azurin (including the OmpA signal sequence for periplasmic secretion) was amplified by PCR from a published plasmid 7 , followed by cloning on pSB19-PapRS#34

Kageyama S, Ii H, Taniguchi K, Kubota S, Yoshida T, Isono T

Whether weight loss can be maintained with lower doses after patients have sustained a stable reduced weight for several years remains to be determined

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Customized physical therapy programs are essential for strengthening the elbow and preventing recurrence