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Allow alcohol to air-dry completely (30-60 seconds) before proceedingpuncturing while wet can introduce alcohol into the solution

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In another study, Yamashita and Kanki (2017) monitored time-lapse imaging of mitophagy and showed that Dnm1 (homolog of DRP1) like-independent mitochondrial division occurs concomitantly with autophagosome formation, which calls into question the necessity of DRP1 in mitophagy

[DOI] [PMC free article] [PubMed] [Google Scholar] 110.Zhang J., Wang X., Guan B., Wang X., An X., Wang T., Chen X., Zhao L., Jia J., Song L., et al

The cellular fluorescence was quantified using the ImageJ software, after background subtraction for each image