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doi: 10.1016/j.addr.2008.03.020

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The amplification of miRNA cDNAs was quantified in real-time SYBR Green RT-qPCR reactions according to the recommended two-step cycling procedure (preincubation/activation at 95 C for 2 min followed by 40 cycles of 95 C for 5 s, 60 C for the 30 s)

Lithium protection against glutamate excitotoxicity in rat cerebral cortical neurons: involvement of NMDA receptor inhibition possibly by decreasing NR2B tyrosine phosphorylation