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Colorimetric detection of GSH based on the pFeSAN In the standard procedure, the assays were performed in 1 mL HAc-NaAc buffer (0.1 M, pH 4.0) with different concentrations of GSH (0.05, 0.5, 2.5, 5, 12.5, 25, 50, 100, 150, 200, 250, 500, or 1000 M), where the TMB and pFeSAN concentrations were 1 mM and 20 g/mL, respectively