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Loinard et al

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To generate Rac1-pGEX-6P-1 for bacterial expression, the pGEX-6P-1 vector was digested with BamHI and NotI, the human Rac1 sequence was PCR-amplified from pRK5-myc-Rac1-WT and inserted using Gibson assembly

And when the liver is happy, the rest of you will be, too