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Importantly, commonly used in vitro BBB models, such as transwell systems and endothelial cell lines including RBE4, bEnd.3, and hCMEC/D3 monolayers, have important limitations, as they may overpredict permeability and do not fully recapitulate key in vivo BBB features, including physiological shear stress and multicellular neurovascular architecture (Chaulagain et al., 2023)

Elevated cortisol levels lead to heightened muscle tension and irritability, causing spasms or twitches in various muscles, including the eyelids

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TJs do not influence the permeability of lipophilic molecules, but hydrophilic molecules must traverse this barrier [67]