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Tissue was homogenized by probe sonication (2 10 pulses, 40% power output), and particulate matter was removed by passing samples through 0.4 m syringe filters

But often, a hidden energy drain is behind it: a vitamin B12 deficiency

The organic fractions combined and dried over MgSO 4 , filtered and concentrated under vacuo

Biochim Biophys Acta Gen Subj 1862:24052416 Ralston NV, Blackwell JL 3rd, Raymond LJ (2007) Importance of molar ratios in selenium-dependent protection against methylmercury toxicity

While on the diet, the time needed for cholesterol crystals to form decreased in the placebo and ursodeoxycholic acid groups, but not in the omega-3 group, suggesting fish oil may prevent the increase in gallstone risk seen with low-calorie weight loss dieting (Mendez-Sanchez 2001)