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Pairs of plasmids expressing fusion proteins were co-transformed into Y2HGold, tested for all four reporters ( AUR1-C , MEL1 , ADE2 , and HIS3 ), and the results are presented in Table 2

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developed a cascade-amplified immunoassay for OTA detection based on nanobody-ALP fusion and oxidase-like nanozyme MnO 2 nanosheets, in which the enzymatic product AA could reduce MnO 2 nanosheets into Mn 2+ ions, limiting their oxidase-like activity toward TMB oxidation [169]