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The fractions containing the recombinant proteins were pooled and dialyzed by ultrafiltration to remove salt and loaded onto a DEAE-cellulose column (Sigma Aldrich) equilibrated with a 30 mM Tris-HCl pH 8.0 and 1 mM EDTA buffer
Rabek, Experimental methods in polymer chemistry: applications of wide-angle X-ray diffraction (WAXD) to the study of the structure of polymers, 505 (Wiley-Interscience, Chichester, 1980) C
