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Description
The dual-mutation of the N-fused CPD with L207I/L214A efficiently avoided the undesired cleavage and in-depth studies confirmed the practicality of this strategy in yielding the proteins with the designed N-terminal amino acid

Some background on the lab the elution buffer contained a high concentration of free (reduced) glutathione to displace your target protein from the binding resin
That is standard refrigerator temperature

Chen, J., Jiang, X., Carroll, S

Gene therapies targeting ECM components or the signaling pathways involved in TM cell contractility could restore normal outflow dynamics
