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In RNAi experiments, synchronized N2 at L1 stage were obtained by alkaline hypochlorite treatment of gravid adults and transferred onto NGM plates (containing 2 mM IPTG and 25 mg/mL carbenicillin) seeded with E
2d, left, 3c), and the CH connecting TM2 and TM3 contacts MlaF (Figs

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