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Gene expression was quantified using SYBR green real-time PCR master mix (TOYOBO, Japan) and amplified under the following conditions: 2 min at 95C, 20 s at 95C, and 20 s at 58C, for 40 cycles

In pancreatic ductal adenocarcinoma, ketone metabolism, particularly BHB as an energy source, promotes tumor growth and progression