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If you are uncertain about how to properly handle the product, its misuse could result in adverse reactions

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During autophagy, intracellular proteins and organelles are enwrapped by a doublemembrane structure that is eventually fused with the lysosome for digestion (Fig 5)

After extrusion through 100 nm polycarbonate membrane filters (Whatman) using a Mini-Extruder (Avanti Polar Lipids, Alabaster, AL), the vesicle solutions were tumbled overnight for equilibration before any subsequent experiments