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For site directed mutagenesis studies, amino acids 2-216 of DCAF16 were selected for site saturation library design and cloning into a pRRL-SFFV-EGFP-cpHalo-(GSG)x9-DCAF16 backbone by GenScript

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Right before we came on, I was telling you the FDA has been in our building 5 times in 4 years

Wadamori et al., 1996)

In the course of the present invention, it was found that solvent extraction is suitable for removing sufficient excess maleimide from the alkylation reaction to allow downstream proceedings steps, while at the same time being faster and allowing for easier handling than methods conventionally used for removing excess maleimide in sulfhydryl labelling reactions