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The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

In addition, heparanase expression is high, principally in the glomerulus, 2448 h after LPS treatment, which leads to the glomerular loss of HS and glycosaminoglycans, and is associated with high blood urea nitrogen (BUN), high urinary albumin/creatinine ratio, and glomerular injury (93, 94)

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