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Confluent cell cultures were washed once with PBS, and then incubated for 20 min at room temperature with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei, washed with PBS, permeabilized with 0.1% Triton-X 100 for 30 min, fixed at 37 C for 20 min with 4% Paraformaldehyde in PBS, and blocked with 2% BSA in PBS at room temperature for 20 min

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Thirumoorthi, MD1 Rand Farjo, MD1 Lisa Sturm, MPH, CIC, FAPIC2 Nicole Nomides, MT(ASCP), MS, CIC, FAPIC3 Outbreak Investigation of Staphylococcal Scalded Skin Syndrome in the Neonatal Intensive Care Unit Using Whole Genome Sequencing American Journal of Infection Control Mando Watson , Simon Struthers , Stephen W Turner Lessons learnt (so far) from establishing models of integrated clinical care for children and young people Arch Dis Child Esther J Hawkes 1, Edward T Andrews 2, Mark P Tighe 3 Medicines update: insulin pumps Archives of Disease in Childhood

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