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The establishment of 1919 GBM cells with overexpression of wildtype PSPH and PSPH V116I was confirmed by enhanced expression of PSPH by immunoblot and consecutive upregulation of ser/gly pathway enzyme SHMT2, most pronounced in PSPH V116I cells (Fig

Moreover, glutamine and leucine cooperate to produce -ketoglutarate through glutaminolysis, which ultimately activates mTORC1

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Regulation of protein kinase B

But the crucial point here is that consistency (NOT avoidance) is the key