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doi: 10.1001/jamaneurol.2016.5778

28 Dermatologists should proactively inquire about sunless tanning use when encountering unclear dermoscopic findings or unexplained systemic symptoms to avoid unnecessary biopsies and treatment

The preferred dose may vary based on body weight, skin type, and personal reaction

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HKA (cell line derived from a keratoacanthoma of human skin), Mewo, and G361 (human melanoma cell lines) cells were procured from the Japanese Collection of Research Bioresources (JCRB, Osaka, Japan) and maintained in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific)