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Remaining amino acids were measured by mixing 10 L of the aqueous extract with 90 L 13 C- and 15 N-labeled amino acid standard solution (10 g per mL) and analyzed using an Agilent 1260 HPLC coupled to an API6500 tandem mass spectrometer

A recent study identified Demethylzeylasteral (T-96), a small molecule from Tripterygium wilfordii Hook.f., as a novel inhibitor of leucine-rich pentatricopeptide repeat-containing protein (LRPPRC), which regulates the stability of mitochondrial DNA-encoded mRNA (mt-mRNA) and plays a crucial role in the synthesis of the OXPHOS complex

[DOI] [PMC free article] [PubMed] [Google Scholar] 19.Curran H.V., Freeman T.P., Mokrysz C., Lewis D.A., Morgan C.J.A., Parsons L.H

Matrigel invasion assay 4T1 cells were seeded in the upper chamber of a Transwell insert (8 m pore size) at a density of 5 10 4 cells per well in serum-free medium and starved overnight