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In their proof-of-concept experiment, the NIST researchers grew a mutant strain of yeast cells that could not manufacture their own glutathione in a medium containing the peptide tagged with a nitrogen isotope

Simic, T., Mimic-Oka, J., Ille, K., Dragicevic, D

The bacterial suspension was used to inoculate 200 mL LB/Amp medium, followed by cultivation at 30 C to OD 600 0.4

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1 Introduction Melatonin, a versatile hormone mainly synthesized by the pineal gland, has a unique lipophilic structure that enables it to cross biological membranes, including the blood-brain barrier