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Methods: Authors used a computer-based gene profiling tool, The Connectivity Map, to identify a number of human genes regulated by GHK, relevant to regulation of cell differentiation, apoptosis and stem cell function
Furthermore, the MitoSox-stained cells were counterstained to identify nuclei by using the 4,6-diamidino-2-phenylindole (DAPI) dye

doi: 10.1016/j.clml.2012.11.001
By promoting the expression of GPX4 and GSH, the cellular defense mechanisms of the cell membrane are enhanced, reducing damage from lipid peroxides and maintaining cellular integrity [40]

Pyruvate drives ATP production in the mitochondria by oxidative phosphorylation and other pathways intersecting the TCA cycle (Gray et al., 2014)
