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I wouldn't hesitate to use again, in fact likely going back for another treatment on another facial issue they discussed or if I need a top up after healing

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10.1186/ar3156 85 WeiQ.LiuD.ChuG.YuQ.LiuZ.LiJ.et al (2022)

Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter

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