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TM6 undergoes the characteristic outward rotation that opens the G protein-binding cavity, TM5 repositions to form the lateral wall of this cavity, and the intracellular ends of TM3 and TM7 reorganize to complete the G protein docking surface

Eichenberger, M

& Ferrannini, E

AMG 966 sample material, recombinant human TNF, and recombinant human TL1A were mixed at various molar ratios one day prior to assay execution and incubated at 4C overnight in order to allow immune complexes to form