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Expect a progressive timeline
A second transfection was conducted 24 h after the initial transfection
To confirm this possibility, we investigated the non-K63-linked polyubiquitinated uS10 in the ubi1-4 quadruple deletion strain expressing only the Ub-WT or Ub-K63R mutant ( Ub-WT and Ub-K63R cells) (Finley et al, 1994
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