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397 While some of the notable responses sparked by such heat sensing are behavioral (e.g., seeking a cooler area) or largely supracellular (vasodilation, sweating, arrector pili relaxation), 397 evidence from model organisms indicates that HSF1 signaling may be upstream and/or downstream of noncell-autonomous regulation of the HSR in heterotypic tissues (i.e., signaling between different tissues)

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However, blood spot cards are a highly stable form of storage, with some studies utilizing cards stored for over a decade.[54] In regard to the analysis, measurements below 1 ng/ml were obtained and reported, despite the fact that these measurements fall below the formal limit of detection for the assay, which is a statistical bound on confidence that the measured value is different from zero