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PeerJ (2017) 5:

Similarly, Li et al

The longer this immune response is in place, the more thyroid damage occurs and the greater the likelihood of the person progressing to hypothyroidism, where the thyroid gland becomes so damaged that it is no longer able to make enough thyroid hormone

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After 24 hours of treatment, cells were incubated for 4 hours at 37C with Aquabluer (Boca Scientific, #6015) and fluorescence intensity was measured at 540ex/590em via a SpectraMax M series microplate reader and normalized to the average of control wells