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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)

S., Tuominen R

The Liver The liver is highly metabolic and has many functions, including the detoxification of blood constituents arriving from the digestive tract, production of bile to aid digestion, metabolism of carbohydrates, lipids, proteins, and drugs, blood pH balance, synthesis of plasma proteins, and the storage and synthesis of glycogen and lipids

This is basic chemistry

As shown by studies, NAC not only enhances GSH by supplying cysteine precursors, reverses the oxidative stress state in SLE patients' T cells, and counteracts GSH depletion brought on by mitochondrial dysfunction in SLE patients' T cells (253)