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Flow cytometric staining using 2,7 dichlorofluorescein diacetate (DCFDA) identified elevated reactive oxygen species (ROS) levels among lymphocytes in ME/CFS and LC donors, compared to healthy controls (Figure 1B, 1C)

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The shift was larger than expected, a phenomenon that has been described before and might be due to the interaction of mmPEG with the polyacrylamide matrix 33
