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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

Study results indicate that wound healing in the case of diabetic foot ulcers is a complex and extremely demanding biological and molecular process involving the coordinated actions of many cell types

doi: 10.1016/S0944-7113(00)80065-3

Discussion In this work we revealed that FA alters the GSH:GSSG redox balance, triggers oxidative stress, and causes cytotoxicity in cells and organisms without any deficiency in DNA repair (Figs