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The protein extraction and delipidation process involved adding 450 L of methanol to 100 L of cell homogenate and vortexed, followed by adding 150 L of chloroform
In contrast to inhibiting H 2 S biosynthesis, some studies have addressed the advantage of poisoning cancer cells with high levels of H 2 S, by using donors that release H 2 S in the tumour and somehow block the most important signalling pathways sustaining cancer survival, such as PI3K and MAPK
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