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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

These different results could be accounted for by both the expression of NADPH oxidase induced by KRAS G12D overexpression, which leads to ROS generation, and an increase in NRF2-dependent ROS scavenging in cells expressing physiological levels of KRAS G12D

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Spread the serum over the face and neck
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