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The first-round PCR was performed in five separate 40 l reactions, each containing 2.0 ng of plasmid template DNA, 1 l each of 10 M forward primer KI#403 and 10 M reverse primer KI#404, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no

However, gastrointestinal side effects (nausea, constipation, diarrhea) still occur, typically mild to moderate in severity

Understanding its limitations is as important as understanding its potential

Deep Hydration : Keeps skin moisturized and prevents dryness at every step
