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AGOmiRNA complexes for in vitro co-IP assays Plasmids expressing AGO2 variants were generated from the pcDNA3.3-3xFLAG-SUMO Eu1 -HsAGO2 plasmid (Addgene, 231372)

Pyruvate kinase mRNA splicing assay Two microgram of total RNA was extracted from primary neurons or astrocytes using Trizol reagent

Conclusion This work reported a novel core-shell DNA self-assembly catalyzed by thiol-disulfide exchange reactions, which could realize target-initiated hybridization chain reaction for signal amplification and molecules gathering

doi: 10.1111/j.1742-4658.2008.06488.x

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