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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel
Here, we conduct single-cell transcriptomic sequencing of islets from male GCGR knockout (GCGR-KO) mice
Nature 485 , 635641 (2012)
NAC provides cysteine, one of the three amino acids that makes up GSH
To screen which candidate genes may be responsible for the healthspan improved by mitochondrial translation inhibition, E
