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Finally, Zhou et al

The reaction between DSF and Cu ions forms a copper diethyldithiocarbamate complex (Cu(DDC) 2 also known as CuET) which is the active, potent anticancer ingredient through inhibition of NF-B and ubiquitin-proteasome system as well as alteration of the intracellular reactive oxygen species (ROS)

Although this method has advantages such as low detection limits and low matrix background, the original CNTs have poor solubility, making them difficult to disperse in aqueous systems and deposit onto sample targets to form a uniform layer

FIGURE 3 In the first step, the thiolate of glutathione acts as a base and deprotonates carbon C4

Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark
