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the mixture was centrifuged at 800 g for 10 min to remove the aqueous phase, and the samples were dried with nitrogen

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4 Revisiting immunotherapy for prostate cancer 4.1 Advancing immune checkpoint blockade therapy with peptide-based innovations Immunotherapy for solid tumors primarily leverages inhibitors targeting programmed cell death-1 (PD-1)/programmed cell death ligand-1 (PD-L1) and cytotoxic T lymphocyte antigen-4 (CTLA-4), collectively known as ICB (Sun et al., 2023a)

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