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DiI and DiO staining Approximately 200 L4-stage worms were stained with either with red DiI (1,1-Dioctadecyl-3,3,3,3-Tetramethylindocarbocyanine Perchlorate, Life Technologies) or green DiO (3,3-Dioctadecyloxacarbocyanine Perchlorate, Life Technologies) in 1.5 mL Eppendorf tubes on a rotator for 3 h

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