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RT-qPCR was performed using Fluidigm 96x96 Dynamic Array Integrated Fluid Circuits (IFC), primed with the IFC Controller HX (Fluidigm) according to the manufacturers guidelines

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Whereas, when glucose is processed through glycolysis, fructose-6-phosphate (F6P) is generated, and F6P interacts with GKRP, promoting its association with GCK, and inhibiting GCK kinase activity (333)

Nevertheless, the most commonly used questionnaire for investigating sexual function and especially penile erectile function is the IIEF, a validated questionnaire currently used in the majority of clinical trials, with the peculiarity, respect to different questionnaires, not only of distinguishing between presence or absence of ED, but also of grading the severity of ED, in clinical setting [30]

Additionally, the relevance of these in vitro findings is uncertain for patients receiving maximally suppressive antiretroviral therapy