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Research protocols employ diverse approaches including isolated mitochondria preparations, permeabilized muscle fiber respirometry, stable isotope tracers for metabolic flux analysis, cellular bioenergetics assays (Seahorse), and in vivo metabolic phenotyping in rodent models

The time to develop this scar and the degree to which it occurs varies from patient to patient

S., Murad, H

Acknowledgements This work was supported by the Singapore National Medical Research Council (R-174-000-137-275), the Jay and Betty Van Andel Foundation (H Eric XU and Karsten MELCHER), Amway (China) Limited (H Eric XU), and the National Institutes of Health 5R01DK071662-08 (H Eric XU) and GM102545 and GM104212 (Karsten MELCHER)

24 Older adults are particularly vulnerable due to age-related decreases in stomach acid production, with B12 deficiency prevalence reaching 6% in those 60 years and 20% in those 85 years