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The tissues were embedded in OCT compound (Sakura Finetek Japan, Tokyo, Japan), frozen at -80C, and sectioned into 7-m thicknesses in a cryostat on HistoBond adhesive microscope slides (Marienfeld-Superior, Lauda-Knigshofen, Germany)
The compounds don't compete for binding sites, don't share metabolic pathways during clearance, and don't modulate the same downstream signaling cascades
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Some people also consider it because they do not tolerate stimulant-based weight-loss products well or are not candidates for GLP-1 medications
