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Data were collected from at least 10,000 cells

Consequently, scientific research has increasingly emphasized the crucial role of a balanced diet in maintaining human health ( However, reported benefits are not uniform across all trials or populations, and effect sizes are often modest, highly strain-specific, and influenced by background diet and host factors, which complicates broad generalization of these findings ( The gut microbiota, the intricate assembly of microorganisms inhabiting the gastrointestinal (GI) tract, assumes a critical role in essential physiological functions, including intestinal development, energy regulation, nutrient absorption, and immune modulation ( This review is expected to clarify the mechanisms underlying the beneficial and nutritional functions of probiotics and to guide the development of sophisticated, next-generation probiotic (NGPs) formulations