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Patients should be alert for: Allergic reaction: Rash, itching, swelling (especially of face/tongue/throat), dizziness, or trouble breathing

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Result: In the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM -ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents