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It was found that reducing Mfrn1/2 in mitochondria reduced the mitochondrial iron content, oxygen consumption rate, and ATP level in fat cells, which lead to a reduction in lipogenic gene expression and lipid synthesis during lipogenic differentiation [51]

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The unspecific proteins were washed in 10 mM NaP i at pH 8, 150 mM NaCl, 1 mM GSH, 10% glycerol, 50 mM imidazole, and 0.1% reduced Triton X-100 (Sigma) and rat MGST1 protein was eluted in 350 mM imidazole

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doi:10.1007/s12011-020-02548-3